RNA
E21

Part:BBa_K273028

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-18)

E21

Antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is dihydrolipoyl transacetylase E2 (EC 2.3.1.12). Antisense RNA approach #1 for this particular enzyme.

Will be transcribed together with the BioBrick interface and may affect the performance of the antisense RNA molecule.

EXZ
EX: E2 enzyme subunit of the PDC complex
Z: # of antisense RNA approach

Usage and Biology

Antisense RNA function supposed to fuction through either Shine Dalgarno blocking and/or RNA elongation blocking.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None