RNA
E1B4

Part:BBa_K273027

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-18)

E1B4

Antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is pyruvate dehydrogenase E1 (EC 1.2.4.1), in partuclar the beta subunit (B). Antisense RNA approach #4.

Will be transcribed together with the BioBrick interface and may affect the performance of the antisense RNA molecule.

EXYZ
EX: E1 enzyme subunit of the PDC complex
Y: a=Alpha subunit of the particular enzyme subunit
b=beta subunit of the particular enzyme subunit
Z: # of antisense RNA approach

Usage and Biology

Antisense RNA function supposed to fuction through either Shine Dalgarno blocking and/or RNA elongation blocking.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7


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Categories
Parameters
None