Composite

Part:BBa_K273015

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-17)


PLlac - RBS - pdc - RBS - adh2(Y) - Ter

This construct encodes for an ethanol producing pathway. The pathway uses pyruvate as a precursor.

We used this construct for testing and characterization purposes in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
    Illegal AgeI site found at 1243
    Illegal AgeI site found at 1621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 130
    Illegal SapI site found at 544
    Illegal SapI site found at 1207


[edit]
Categories
Parameters
None