Composite
Part:BBa_K273015
Designed by: Karl Brune Group: iGEM09_Uppsala-Sweden (2009-10-17)
PLlac - RBS - pdc - RBS - adh2(Y) - Ter
This construct encodes for an ethanol producing pathway. The pathway uses pyruvate as a precursor.
We used this construct for testing and characterization purposes in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1096
Illegal AgeI site found at 1243
Illegal AgeI site found at 1621 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 130
Illegal SapI site found at 544
Illegal SapI site found at 1207
[edit]
Categories
Parameters
None |