Intermediate

Part:BBa_K273014

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-17)


RBS - pdc - RBS - adh2(Y) - Ter

Construct intermediate. Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191 Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from S. cerevisiae


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1033
    Illegal AgeI site found at 1180
    Illegal AgeI site found at 1558
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 67
    Illegal SapI site found at 481
    Illegal SapI site found at 1144


[edit]
Categories
Parameters
None