J23108 - supD - B1006
Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions.
Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 77
- 1000Illegal BsaI site found at 80