Part:BBa_K2725012
P1003 Cassette with 5 amber stop codons
A P1003 kanamycin resistance cassette with 5 serine -> amber stop codon (TAG) substitutions: S36Amb, S133Amb, S154Amb, S200Amb, S240Amb. This gives kanamycin resistance when co-transformed with BBa_K2725016. This part demonstrates functionality of the semantic containment system for preventing horizontal gene transfer. This part is an improvement upon BBa_K914009 (https://parts.igem.org/Part:BBa_K914009).
Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin.
Figure 2. Graph showing the probability of a read-through (and therefore expression) of a gene with number of amber STOP codons within the coding sequence of the gene. When there are 5 amber STOP codons in the coding sequence 1 in every 1010 translation attempts may result in expression of the gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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