Has a MicC construct targeted to knockdown mCherry and an mCherry gene under the control of an arabinose promoter. The intended effect was as a control to demonstrate that our MicC constructs were successful at knocking down genes, however it was observed that mCherry fluorescence increased for this part compared to control mCherry fluorescence levels. We theorised that this was due to a mutation in the terminator, leading to mCherry being transcribed from both promoters. A failure to terminate the transcript might also deform this siRNA, preventing it from knocking down mCherry.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 1398
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1233
- 1000Illegal SapI site found at 1215