Part:BBa_K2725004
FabV Under Low Constitutive Expression Cassette
FabV is an enoyl acyl-carrier-protein reductase of Vibrio, which is involved in type 2 fatty acid synthesis. It is a target for the biocide triclosan, which suppresses growth of E. coli DH5-alpha on agar plates at concentrations above 1 μM. When heterologously expressed in E. coli, this confers resistance to as it binds to triclosan, so the enoyl ACP reductase of E. coli (FabI) is free to function. FabV was expressed under K314101, a low constitutive expression cassette, and growth was observed on 1 μM triclosan plates, as shown in the following figure: http://2018.igem.org/File:T--Edinburgh_UG--BBa_K2725004.jpg#file BBa_K2725004 is our recommended part for conferring triclosan resistance, as it allows selection on 1 μM triclosan plates and would be less of a metabolic burden than BBa_K2725003.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]
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