Composite
HCEC:FabV

Part:BBa_K2725003

Designed by: Ben Astles   Group: iGEM18_Edinburgh_UG   (2018-10-11)


FabV Under High Constitutive Expression Cassette

FabV is an enoyl acyl-carrier-protein reductase of Vibrio species, which is involved in type 2 fatty acid synthesis. It is a target for the biocide triclosan, which suppresses growth of E. coli DH5-alpha on agar plates at concentrations above 1 μM. When heterologously expressed in E. coli, this confers resistance to as it binds to triclosan, so the enoyl ACP reductase of E. coli (FabI) is free to function. FabV was expressed under K314100, a high constitutive expression cassette, and growth was observed on 1 μM triclosan plates, as shown in the following figure: http://2018.igem.org/File:T--Edinburgh_UG--BBa_K2725003.jpg#file

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 527
    Illegal suffix found in sequence at 1752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 527
    Illegal NheI site found at 470
    Illegal NheI site found at 493
    Illegal SpeI site found at 1753
    Illegal PstI site found at 1767
    Illegal NotI site found at 533
    Illegal NotI site found at 1760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 527
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 527
    Illegal suffix found in sequence at 1753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 527
    Illegal XbaI site found at 542
    Illegal SpeI site found at 1753
    Illegal PstI site found at 1767
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None