Coding
FabV

Part:BBa_K2725002

Designed by: Ben Astles   Group: iGEM18_Edinburgh_UG   (2018-10-11)


FabV coding sequence

FabV is an enoyl acyl-carrier-protein reductase of Vibrio, which is involved in type 2 fatty acid synthesis. It is a target for the biocide triclosan, which suppresses growth of E. coli DH5-alpha on agar plates at concentrations above 1 μM. When heterologously expressed in E. coli, this confers resistance to as it binds to triclosan, so the enoyl ACP reductase of E. coli (FabI) is free to function. This part was characterised by showing growth on 1 μM triclosan agar plates when expressed under a high constitutive expression cassette and a low constitutive expression cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None