Composite

Part:BBa_K2717015

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


GFP-TGATG-T7 RNA Polymerase-GDH-v5 tag-his tag

The vector has GFP-TGATG-T7 RNA Polymerase , T7 promoter and gdh, which can increase the growth rate of the strain by positive feedback and delay the senescence of the strain.

Function: Positive feedback adjustment can accelerate the growth rate of the strain and the yield of the product, as well as delay the degradation in the military.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5252
    Illegal AgeI site found at 6144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6126
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None