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Part:BBa_K2712000

Designed by: Sean Ryan   Group: iGEM18_UIOWA   (2018-10-09)


3-HP Inducible Promoter (PmmsA)

This is a promoter that was selected for use in Bacillus subtilis. It originates from Pseudomonas denitrificans. This promoter is regulated by the Lys-family regulator protein encoded by the mmsR biobrick and is intended for use as a biosensor for 3-hydroxyproponic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


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