Device

Part:BBa_K271001

Designed by: Hanseong Roh   Group: iGEM09_KU_Seoul   (2009-10-20)

Heavy-metal detector

The promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance(mer) operons, including a long spacer region containing an inverted repeat sequence.


Compatibility

Chassis : DH5α

Plasmid : pSB3T5


Experiments

Measure of expression by fluoresence

http://2009.igem.org/Team:KU_Seoul/Result



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1558
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1558
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1558
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1558
    Illegal AgeI site found at 1439
    Illegal AgeI site found at 1551
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694


[edit]
Categories
Parameters
None