Part:BBa_K2707004
cob
Usage and Biology
cob encode cytochrome b in mitochondrion. Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.
Characterization
It's too hard to characterize this part in mitochondrial due to the difficulty of transfering DNA into mitochondrial.However, we try our best to characterize this part. Mitochondrial genome's GC content is very low. When we construct this genome, We found that DNA fragments with low GC content replicated in cells and cause cytotoxicity. So We constructed this part on the pSB1C3 backbone and examined the growth curve of this part in E.coli replication. Used to detect whether a large amount of replication of the gene is toxic to cells.
Figure 1. Growth curve of E.coli which was transfered into cob gene.
Figure 2. The most left lane is marker whose length has been showed in the figure. The lane labeled “1” is segment of gene named COB ,which is 1158bp as showing in the figure.
Conclusion
We can see two curve is similar. we can infer that this gene is not toxic to cells when it replication in a high copy mode.
Others
The chassis is DH5alpha.
Blank is DH5alpha which was transfered into pSB1C3 circle plasmid
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 847
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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