Coding

Part:BBa_K2706001

Designed by: Elizabeth Chamiec-Case   Group: iGEM18_UConn   (2018-10-09)


tesA (L109P) with pTac, rbs (BBa_B0034), and terminator (BBa_B0015)

With the L109P amino acid mutation, 'tesA (L109P) can efficiently convert fatty acyl-ACPs into free fatty acids that are then viable for a short-chain alkane synthesis pathway. This addition of function to the 'tesA gene is an improvement upon the 'tesA part (BBa_K612002) created by the 2011 Alberta iGEM team. The gene is accompanied by promoter pTac, rbs BBa_B0034, and terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None