Plasmid_Backbone
pAGM9121

Part:BBa_K2703011

Designed by: Aurélie Bouin, Victor Sayous   Group: iGEM18_Sorbonne_U_Paris   (2018-10-10)


pAGM9121

pAGM9121 is a high copy number plasmid carrying Spectinomycin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2295
    Illegal XbaI site found at 2268
    Illegal PstI site found at 2256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2295
    Illegal PstI site found at 2256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2295
    Illegal BamHI site found at 2274
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2295
    Illegal XbaI site found at 2268
    Illegal PstI site found at 2256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2295
    Illegal XbaI site found at 2268
    Illegal PstI site found at 2256
    Illegal NgoMIV site found at 932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2047
    Illegal BsaI.rc site found at 2649


[edit]
Categories
Parameters
None