Coding

Part:BBa_K270005

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-10-19)

XylR

This is a regulatory protien that is under constituative expression of the Pr promoter. When no toluene is present it represses the Pr promoter and when toluene is present it binds with the toluene to activate the Pu promoter.

For more information about this part see Bba_K270001: Pr XylR


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 466
    Illegal PstI site found at 643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
    Illegal BglII site found at 1548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal NgoMIV site found at 252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


[edit]
Categories
Parameters
None