Reporter

Part:BBa_K270003

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-10-19)

Pu GFP

PURPOSE

This device will characterize the activity of the Pu promoter using a GFP fluorescense signal.


CONSIDERATIONS WHEN USING Pu GFP When working in species other than P. putida mt-2, the Pr XylR regulator part is needed in order to activate the Pu promoter in the presense of toluene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 295
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1013


[edit]
Categories
Parameters
n/aPu GFP