Plasmid_Backbone

Part:BBa_K270002

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-10-19)

Leucine Landing Pad

This was a convenient biobrick compatable vector that we already had on hand to hold our Pr XylR part. It is also useful for three way ligations because it has both ampicillian and choloramphenicol resistance markers.


For more on landing pads see our past IGEM projects:

[http://synthbio.engin.umich.edu/wiki/index.php/BioBrick_Landing_Pad Michigan IGEM 2007]

[http://2008.igem.org/Team:Michigan/Project/LandingPads Michigan IGEM 2008]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5821
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5827
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5821
    Illegal BamHI site found at 4647
    Illegal XhoI site found at 42
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5821
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5821
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5836
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2355
    Illegal SapI site found at 262
    Illegal SapI site found at 4473
    Illegal SapI.rc site found at 1333
    Illegal SapI.rc site found at 3437


[edit]
Categories
Parameters
None