Plasmid_Backbone
Part:BBa_K270002
Designed by: Ann Lesnefsky Group: iGEM09_Michigan (2009-10-19)
Leucine Landing Pad
This was a convenient biobrick compatable vector that we already had on hand to hold our Pr XylR part. It is also useful for three way ligations because it has both ampicillian and choloramphenicol resistance markers.
For more on landing pads see our past IGEM projects:
[http://synthbio.engin.umich.edu/wiki/index.php/BioBrick_Landing_Pad Michigan IGEM 2007]
[http://2008.igem.org/Team:Michigan/Project/LandingPads Michigan IGEM 2008]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5821
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5827 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5821
Illegal BamHI site found at 4647
Illegal XhoI site found at 42 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5821
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5821
Plasmid lacks a suffix.
Illegal XbaI site found at 5836
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2355
Illegal SapI site found at 262
Illegal SapI site found at 4473
Illegal SapI.rc site found at 1333
Illegal SapI.rc site found at 3437
[edit]
Categories
Parameters
None |