Composite

Part:BBa_K2690000

Designed by: David Doyle   Group: iGEM18_SUIS_Shanghai   (2018-10-09)


PvsA-E (Vibrioferrin Biosynthetic gene cluster)

This is a bio synthetic gene cluster for the synthesis and excretion of the siderophore vibrioferrin. The cluster contains 5 coding sequences pvsA-E originally from the marine bacterium Vibrio parahaemolyticus. This composite part does NOT contain the ferric uptake regulator but rather contains an inducible T7 promoter region.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1888
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 398
    Illegal NgoMIV site found at 2017
    Illegal NgoMIV site found at 3729
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 5888
    Illegal AgeI site found at 3792
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696
    Illegal SapI.rc site found at 98
    Illegal SapI.rc site found at 7261


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Categories
Parameters
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