Part:BBa_K2683022
Tryptophan tRNA Synthetase (TrpRS) in pSb1C3
Tryptophan tRNA synthetase is the protein responsible for attaching Tryptophan onto its tRNA to form an aminoacyl-tRNA [1]. This part is codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443019).
This part was improved by the placing the part into the pSB1C3 plasmid.
Figure 1- An overexpression of TrpRS in pSB1C3 run on a 12% SDS-PAGE gel. Samples were taken at point of induction and in 1 hour increments thereafter. The induced protein can be seen at the 39kDa range.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 705
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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