Part:BBa_K2683021
Serine tRNA Synthetase (SerRS) in pSB1C3
Serine tRNA synthetase is a protein responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA [1]. The part is codon optimized for use in Escherichia coli , contains a C-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team 2017 (http://2017.igem.org/Team:Lethbridge). The part was characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443017).
The part was improved by placing it within the pSB1C3 plasmid.
Figure 1- The overexpression of SerRS protein run on a 12% SDS-PAGE. Samples were taken at point of induction and in 1 hour increments thereafter. The Induced protein expression can be seen at the 50kDA range.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 172
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