Composite

Part:BBa_K2683019

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)


Lysine tRNA Synthetase (LysRS) in pSB1C3

Lysine tRNA synthetase is a protein responsible for attaching lysine onto its tRNA to form an aminoacyl-tRNA [1]. This part is codon optimized for use in Escherichia coli , contains a N-terminal hexahistidine tag with a serine glycine linker and has a T7 Promoter, medium strength RBS and double terminator. These designs were originally done by the Lethbridge iGEM team in 2017 (http://2017.igem.org/Team:Lethbridge).

This part was improved by LEthbridge iGEM 2018 by placing the part in the pSB1C3 plasmid.

T--Lethbridge--LysRSOE.PNG

Figure 1- Overexpression of LysRS protein run on a 12% SDS-PAGE gel. Samples were taken at point of induction and in 1 hour increments after. The induced samples can be seen at about the 60kDa mark.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1459
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733


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