Composite

Part:BBa_K2683015

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-08)


DecS134C codon optimized for E.coli

DecS134C is a mutated form of a protein originally from the phage L genome. It forms a trimer dimer when presented with protein nanocompartments as seen in previous literature [1]. The mutation of serine to cysteine causes the interactions between the other DecS134C which causes connections of dimers that can form higher order structures[1]. This part is optimized for expression in E.coli and has a hexahistidine tag on the N-terminus for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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