Part:BBa_K2683013
Arc Protein
The Arc protein is originally found in mammalian brain tissue. It is known to form capsid structures that can delivery RNA from one cell to the other; specifically neuronal cells. Arc has shown to have similar attributes to retroviral Gag proteins [1]. The sequence given is based on the gene found in mouse. This part has been Codon optimized for expression in E.coli .
In order to highly characterize the Arc protein, we have used a series of experiments including protein purification and Transmission Electron Microscopy.
Figure 1- An 15% SDS-PAGE gel showing the purification of the arc protein. The protein was purified by running it through a sucrose cushion, dialyzing against 1XPBS and running it through a size exclusion column. The purified protein can be found at the size of about 45kDa.
Figure 2- A chromatogram of the purification of Arc protein using size exclusion chromatography. Elutions were collected based on the peaks and used for further experiments and gel confirmation
Figure 1- The transmission electron microscopy image of the arc protein. The diameter of the capsid in about 30nm.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 603
- 1000COMPATIBLE WITH RFC[1000]
| None |