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Part:BBa_K2683010

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-08)


Truncated Scaffolding protein 141 from P22 fused with Super folded GFP

The Scaffolding protein from P22 facilitates the formation of capsids. Previous literature has shown that the Scaffolding protein can be truncated resulting in a protein with the remaining amino acids in positions 141-303 (SP141) [1]. Delivery of proteins has been shown to occur with the fusion of SP141 [1,2]. As a proof of concept for encapsulation and delivery applications we have fused it with super-folded GFP. This fusion protein has been codon optimized for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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