Coding

Part:BBa_K2683008

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)


truncated scaffolding protein 141 from P22 fused with Cas9

SPCas9 is a cargo for the P22 capsid protein from P22 bacteriophage. The truncated scaffolding protein is originally found in the P22 bacteriophage genome and it helps with forming P22 capsids. When fused with the Cas9 it can cause formation of the P22 around the Cas9, effectively encapsulating it [1]. This part has a hexahistidine tag on the N-terminus of the construct.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1922
    Illegal SpeI site found at 547
    Illegal PstI site found at 22
    Illegal PstI site found at 514
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1922
    Illegal NheI site found at 1681
    Illegal SpeI site found at 547
    Illegal PstI site found at 22
    Illegal PstI site found at 514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1922
    Illegal BamHI site found at 3960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1922
    Illegal SpeI site found at 547
    Illegal PstI site found at 22
    Illegal PstI site found at 514
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1922
    Illegal SpeI site found at 547
    Illegal PstI site found at 22
    Illegal PstI site found at 514
    Illegal AgeI site found at 397
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None