Coding

Part:BBa_K2683004

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-07)


Green Fluorescent protein (GFP) with Anionic tag

GFP is a fluorescent protein that is derived from jellyfish. As a proof of concept for transfection or transformation applications using Protein nanocompartments (http://2018.igem.org/Team:Lethbridge) an anionic tag was added onto the C-terminus of the protein to aid encapsulation[1]. As well, a hexa-Histidine tag was added onto the N-terminus for purification purposes. This part is also codon optimized for expression in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None