DNA

Part:BBa_K2680553

Designed by: Stephanie Do   Group: iGEM18_William_and_Mary   (2018-10-16)


UNS 1 Sequence, from Torella et al., 2013

UNS 1 Sequence, from Torella et al., 2013

This is Unique Nucleotide Sequence 1, (UNS 1), from Torella et al., 2013. This UNS1 sequence is necessary for our 3G assembly, a method that combines Gibson and Golden Gate. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].


The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None