Coding

Part:BBa_K2680532

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-13)


sfGFP with BsaI sites removed

sfGFP with BsaI sites removed.

sfGFP is a "robustly folded version of GFP [...] that folds well even when fused to poorly folded polypeptides."1 Compared to other GFP variants, sfGFP "shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics."1

References

1Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24(1), 79–88. https://doi.org/10.1038/nbt1172

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


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Categories
Parameters
None