Part:BBa_K2680530
B0033m
B0033m is a variant of B0033 designed for use in Moclo type assemblies (has added spacers on the side). B0033 is a weak RBS, from the Weiss series. B0033m was designed in Iverson et al. 2015.1
References
1Iverson, S. V., Haddock, T. L., Beal, J., & Densmore, D. M. (2015). CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synthetic Biology, 5(1), 99-103. doi:10.1021/acssynbio.5b00124
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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NYU Abu Dhabi 2024 Characterization
Group:
NYU-Abu-Dhabi 2024 BBa_B0033_m0 was assembled using Golden Gate Assembly, with eYFP as a reporter to measure its ribosome binding site (RBS) activity via fluorescence at two temperatures: 28°C and 37°C. In the RBS activity graph, BBa_B0033_m0 is represented by the green lines. At 37°C (solid green line), BBa_B0033_m0 started with low activity, which then gradually increased before stabilizing and slightly declining towards the end of the time course. At 28°C (dashed green line), BBa_B0033_m0 followed a similar trend, starting with low activity, then steadily rising and reaching a peak before decreasing slightly. The activity at 28°C was consistently higher than at 37°C, indicating that BBa_B0033_m0 performs better at lower temperatures than under heat stress. 
Figure 1. RBS activity of BBa_B0033_m0 at 28°C and 37°C. </center>
Figure 1. RBS activity of BBa_B0033_m0 at 28°C and 37°C. </center>
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