Part:BBa_K2680529
BCD12
Bicistronic design medium-strength RBS from Mutalik et al. 2013.1
Our team characterized the BCD12 RBS in the genetic context of luxI gene.
Measurment
We characterized this RBS in the genetic context of the LuxI coding sequence. We built a fusion of the first 36 nucleotides of the LuxI synthase with sfGFP to characterize the relative strength of our deployed RBS in the context of the LuxI coding sequence. To quantify this output we measured the fluorescence using microplate reader FlexStation3 (Molecular Devices) with excitation wavelength 485nm and emission wavelength 510nm. We conducted measurements in different time points after the induction with aTc, using different concentrations of the inducer.
'Figure 1: Characterization of BCD12 RBS in the genetic context of luxI'
'Figure 2: Characterization of BCDs in the genetic context of luxI'
'Figure 3: Characterization of all the RBS our team tested in the genetic context of luxI'
References
1Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q.-A., … Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature Methods, 10(4), 354–360. https://doi.org/10.1038/nmeth.2404
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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