Regulatory

Part:BBa_K2680527

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-13)


pLsrA promoter

pLsrA promoter. LsrA is one of four genes in the lsr quorum-sensing operon that aids in the uptake of Autoinducer-2 (AI-2) into the cell. Specifically, "lsrA encodes the ATPase that provides energy for AI-2 transport." Phosphorylated AI-2 (phospho-AI-2) induces the lsr operon by binding to LsrR, which represses the operon.1

William and Mary iGEM encountered this part through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki].

References

1Xavier, K. B., & Bassler, B. L. (2005). Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. Journal of Bacteriology, 187(1), 238–248. http://doi.org/10.1128/JB.187.1.238-248.2005

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None