Primer

Part:BBa_K2680515

Designed by: Xiangyi Fang   Group: iGEM18_William_and_Mary   (2018-07-31)


WM_Pad insert amplification primer fwd

This primer (along with BBa_K2680516) can be used to amplify the insert of any of our Pad1 flanked level 0 parts.

Thermodynamic Info

No hairpin concerns
No homodimer concerns
No heterodimer concerns with BBa_K2680516
T_m in Q5 HiFi 2X MasterMix (from NEB): 64C
Anneal with BBa_K2680516 at 64C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None