Composite

Part:BBa_K2680406

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G S. pyogenes tracrRNA terminator

S. pyogenes tracrRNA terminator flanked by prefix sticky end D and suffix sticky end E (as well as spacers) in a 3G-compatble backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 125


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