Part:BBa_K2680405

3G ECK120033736

Transcriptional terminator ECK120033736 flanked by prefix sticky end D and suffix sticky end E (as well as spacers) in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

ECK120033736 is the fifth strongest natural transcriptional terminator characterized in Chen et. al 2013 (see supplementary figure 1). "Terminator strength (TS) was determined from a simple assay that compares the expression of two fluorescent reporters, one placed before and one after the terminator."¹ Marked as recombination-resistant in a spreadsheet from Christopher Voigt lab.²

References

¹Chen, Y. J., Liu, P., Nielsen, A. A., Brophy, J. A., Clancy, K., Peterson, T., & Voigt, C. A. (2013). Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nature methods, 10(7), 659.

²Voigt Lab Terminator Plasmids Available from Addgene. (n.d.). Retrieved October 14, 2018, from https://www.addgene.org/synthetic-biology/voigt/terminators/

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 47
  Illegal BsaI.rc site found at 126