Part:BBa_K2680403

3G T500_noGap, short attachment (T11)

T500 terminator variant flanked by prefix sticky end D and suffix sticky end E (as well as spacers) in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

T500 is a strong intrinsic terminator "derived from bacteriophage Φ82".^1,2 Requires hypertranslocation by RNA polymerase.²

References

¹Shin, J., & Noireaux, V. (2010). Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. Journal of Biological Engineering, 4(1), 8. doi:10.1186/1754-1611-4-8

²Greenblatt, Jack. F. (2008). Preview: Transcription termination: pulling out al the stops. Cell, 132, 918. doi:10.1016/j.cell.2008.03.003 Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 47
  Illegal BsaI.rc site found at 102