Part:BBa_K268027
YDL205C -> GFP
~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YDL205C gene. For transformation in S. cerevisiae only, this part will swap out the YDL205 gene and replace it with a Kan marker+GFP coding region downstream of the YDL205C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.
This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers: Foward: 62-mer CACAGAAAAAAAGAAAATATAATAAATTAGTATTGCGATATGcggatccccgggttaattaa Reverse: 60-mer CGCAAAACAGAGGGTTCGAAGGAAAACAGGAAACCTCTACgaattcgagctcgtttaaac
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2461
Illegal BglII site found at 1015
Illegal BamHI site found at 44 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2461
Illegal PstI site found at 1225 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 704
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