Composite

Part:BBa_K2680265

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G sfCFP-pdt3

3G sfCFP-pdt3 coding sequence flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Superfolding cyan fluorescent protein variant. Similar to BBa_K1093002.

"Superfolder Cyan Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to CFP it displays improved stability and strong cyan fluorescence. Excitation peak: 425 nm Emission peak: 475 nm" 1

References

1Miścicka, A. (2013, September 20). Part:BBa_K1093002. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K1093002


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 861
    Illegal SapI.rc site found at 67


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Categories
Parameters
None