Composite

Part:BBa_K2680259

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G TetR-ssrA

TetR-ssra flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Tetracycline Repressor proteins give tetracycline-resistance to bacteria. This TetR variant has an ssra degradation tag. It is similar to BBa_C0040, though lacking a barcode. "TetR binds to the pTet regulator (Part:BBa_R0040). [http://openwetware.org/wiki/ATc aTc (anhydrotetracycline)] binds to TetR and inhibits its operation."1

References

1Rhee, J., Tao, C., Thomson, T., & Waldman, L. (2003, January 31). Part:BBa_C0040. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_C0040

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 720


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