Part:BBa_K268022

YLR346C -> GFP

~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YLR346C gene. For transformation in S. cerevisiae only, this part will swap out the YLR346 gene and replace it with a Kan marker+GFP coding region downstream of the YLR346C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.

This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers: YLR346Cdel-F: 62-mer GGGGCACAAATAACAAAGGATTATTTATCACCTTTAATAATGcggatccccgggttaattaa YLR346Cdel-R: 62-mer GATATGAGGGGACGATAGCTTAACATGATTGTGACACAAACTgaattcgagctcgtttaaac

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2461
  Illegal BglII site found at 1015
  Illegal BamHI site found at 44
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2461
  Illegal PstI site found at 1225
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 704