Composite

Part:BBa_K2680203

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G B0033m

B0033m flanked by prefix sticky end B and suffix sticky end C (as well as spacers) in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

B0033m is a variant of B0033 designed for use in Moclo type assemblies (has added spacers on the side). B0033 is a weak RBS, from the Weiss series. B0033m was designed in Iverson et al. 2015.1

References

1Iverson, S. V., Haddock, T. L., Beal, J., & Densmore, D. M. (2015). CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. ACS Synthetic Biology, 5(1), 99-103. doi:10.1021/acssynbio.5b00124

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 83


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