Part:BBa_K268019

YOR186W -> GFP

~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YOR186W gene. For transformation in S. cerevisiae only, this part will swap out the YOR186 gene and replace it with a Kan marker+GFP coding region downstream of the YOR186W promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.

This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers: YOR186WCdel-F: 64-mer GTTTACTCCTTCTCTCAAATACTACGTAACAGATCTTATTGATGcggatccccgggttaattaa YOR186Wdel-R: 66-mer GTTTTAAATGAGAAAAGCAGCTTCTTTTCCTTTCTTCCACGTAGTAgaattcgagctcgtttaaac

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2463
  Illegal PstI site found at 1227
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2463
  Illegal PstI site found at 1227
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2463
  Illegal BglII site found at 31
  Illegal BglII site found at 1017
  Illegal BamHI site found at 46
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2463
  Illegal PstI site found at 1227
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2463
  Illegal PstI site found at 1227
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 706