Part:BBa_K268018

YOR049C -> GFP

~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YOR049C gene. For transformation in S. cerevisiae only, this part will swap out the YOR049 gene and replace it with a Kan marker+GFP coding region downstream of the YOR049C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.

This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers: YOR049Cdel-F: 60-mer GAAGCAGCAGCTAACAGTAAATTCCCTTTATGGTGGTATGcggatccccgggttaattaa YOR049Cdel-R: 60-mer CATATGAACGATCAGATTGACATTCAAATTCTACGTATCAgaattcgagctcgtttaaac

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2459
  Illegal PstI site found at 1223
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2459
  Illegal PstI site found at 1223
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2459
  Illegal BglII site found at 1013
  Illegal BamHI site found at 42
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2459
  Illegal PstI site found at 1223
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2459
  Illegal PstI site found at 1223
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 702