Part:BBa_K268017

YHR139C->GFP

~2.6kbp linear DNA fragment used for genomic integration and expression expression of GFP under the control of the promoter for YHR139C gene. For transformation in S. cerevisiae only, this part will swap out the YHR139C gene and replace it with a Kan marker+GFP coding region downstream of the YHR139C promoter. Promoter shown to be up-related by the neurotoxin paraoxon and/or hydrolysis of paraoxon to p-nitrophenol and diethyl-phosphate.

This part can be retrieved from the yeast genome in which it was integrated through colony PCR of the following primers: YHR139Cdel-F: 65-mer GATTTCGATCAGTTTTCACATCTAAGAACCCAATCAGCTATTATGcggatccccgggttaattaa YHR139Cdel-R: 60-mer ATTTTTTGCTTATTTTCGTAGTACACAACACATAATCATCgaattcgagctcgtttaaac

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2464
  Illegal PstI site found at 1228
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2464
  Illegal PstI site found at 1228
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2464
  Illegal BglII site found at 1018
  Illegal BamHI site found at 47
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2464
  Illegal PstI site found at 1228
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2464
  Illegal PstI site found at 1228
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 707