Composite

Part:BBa_K2680123

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G pTlpA

pTlpA promoter BBa_K2500003 flanked by prefix sticky end A and suffix sticky end B in in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the William and Mary 3G Parts Page.

pTlpA is repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacIQ) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."1 See pTlpAr BBa_K2680124 for the reverse complement of pTlpA (also in 3G compatible form).

References

1Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 109


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