pTlpA promoter BBa_K2500003 flanked by prefix sticky end A and suffix sticky end B in in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the William and Mary 3G Parts Page.
pTlpA is repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. "The TlpA operator is a strong promoter (88-fold stronger than LacIQ) driven by the transcription factor σ. Interestingly, this promoter has bidirectional activity, with identical thermal regulation in both orientations but approximately 200-fold lower maximal expression in the reverse direction."1 See pTlpAr BBa_K2680124 for the reverse complement of pTlpA (also in 3G compatible form).
1Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 41
Illegal BsaI.rc site found at 109