Composite

Part:BBa_K2680121

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G pLsrR

pLsrR promoter flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pLsrR promoter. LsrR represses the lsr operon, a quorum sensing operon that processes Autoinducer-2 (AI-2). When phosphorylated AI-2 (phosphorus-AI-2) binds to LsrR, LsrR deactivates and no longer represses the lsr operon.1

William and Mary iGEM encountered the promoter for lsrA through our collaboration with Virginia iGEM. For more information, visit [http://2018.igem.org/Team:Virginia/Parts Virginia iGEM's wiki].

References

1Xavier, K. B., & Bassler, B. L. (2005). Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. Journal of Bacteriology, 187(1), 238–248. http://doi.org/10.1128/JB.187.1.238-248.2005


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 193


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