Composite

Part:BBa_K2680110

Designed by: Xiangyi Fang   Group: iGEM18_William_and_Mary   (2018-08-02)


3G PlasAM

pLasAM flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pLasAM is the promoter to LasAM, an optimized sensor from Meyer et al (2018).1

References

1Meyer, A. J., Segall-Shapiro, T. H., & Voigt, C. A. (2018). Marionette: E. coli containing 12 highly-optimized small molecule sensors. BioRxiv, 285866. doi:https://doi.org/10.1101/285866

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 133


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Categories
Parameters
None