Part:BBa_K268001
pRS316
Empty plasmid for gene expression in S. cerevisiae. Ura selection in yeast and ampicillan resistance in E. coli. One of a series of pBluescript-based centromere vectors (ATCC 77142 ATCC 77145, ATCC 77157 to ATCC 77158) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4.
Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.8; PvuII--4.2, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416309 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS313, pRS315, pRS316.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041
Illegal NotI site found at 2009 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal BamHI site found at 2029
Illegal XhoI site found at 2080 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2047
Illegal PstI site found at 2041
Illegal NgoMIV site found at 1668 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1079
Illegal BsaI.rc site found at 3453
Illegal SapI site found at 2370
Illegal SapI site found at 4474
Illegal SapI.rc site found at 926
| None |