Plasmid_Backbone

Part:BBa_K268000

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)

pSB6A0

Biobricked plasmid backbone pRS314 with XbaI, PstI and SpeI sites removed from outside the MCS. One of a series of pBluescript based yeast centrometer vectors developed by Sikorski R.S., Hieter P (Genetics 122:19-27(1989)). Confers Trp resistence in yeast and ampicillan resistance in E. coli where it can be transformed and amplified for cloning purposes.



WARNING: THIS PART STILL CONTAINS AT LEAST ONE FORBIDDEN RESTRICTION SITE (XbaI or PstI) IN THE BACKBONE! FOR MORE INFORMATION PLEASE SEE EXPERIENCE SITE. ALSO THE PREDICTION ALGORITHM FOR FORBIDDEN SITES DO NOT WORK PROPERLY IN THIS CASE. AT BASEPAIR 46 there is a NotI RESTRICTION SITE OF RFC10. TEAM: TU_MUNICH 2012

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4793
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 46
    Illegal NotI site found at 4799
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4793
    Illegal BamHI site found at 28
    Illegal XhoI site found at 4760
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4793
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4793
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4808
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3387
    Illegal SapI.rc site found at 2365
    Illegal SapI.rc site found at 4469


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Categories
Parameters
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